Animal Health Division

Mandate :

Development of preventive and control measures, alternative therapies and diagnostics for maintaining and improving health of goats

Patents Granted :

• An antibacterial herbal composition for animals (patent number: 327196 dated 11-12-2019).
• A herb based antibacterial preparation for veterinary use (patent number: 341364 dated 03-07-2020).
• A synergistic anti-bacterial herbal preparation for animals (patent number: 340760 dated 07-07-2020).
• AJAS green: Goat milk based natural herbal beauty soap (patent number: 364566 dated 13-04-2021).
• AJAS: Goat milk based natural beauty soap (patent number: 383967 dated 09-12-2021).
• Method for preparation for herbal anti stressor formulation for goat (patent number: 421306 dated 13-02-2023).
• A formulation having antibacterial herbal extract for animal use (patent number: 425919 dated 20-03-2023).
• AJAS antiseptic: Goat milk based natural herbal antiseptic soap (patent number: 433430 dated 31-05-2023).
• Method for development of herbal immunomodulator composition for goats (patent number: 446708 dated 23-08-2023) .

Commercialized Technologies

• Alquit   :     Herbal ectoparasiticidal drug for animals
• HEALEX-FR   :    Herbal skin antiseptic gel for animals
• Diarrionex-HS   :     Herbal anti-diarrhoeal powder for animals
• Wormolex   :     Herbal anthelminitic bolus for animals
• Megatex   :     Herbal acaricidal liquid/spray for animals
• IMU-4   :    Herbal immunomodulatory bolus for animals
• AJAS   :     Goat milk based herbal fortified soap (three variants)
• Indigenous JD Vaccine (BioJD Gel   and BioJD Oil)   :     It is killed vaccine, and was developed using �Indian Bison Type� genotype of Mycobacterium avium subspecies paratuberculosis strain �S5� of goat origin

ICAR Certified Technologies

• Para-Check Card:   :     An eye mucosa colour based targeted selective treatment chart against haemonchosis in goats
• Sanjeevani:   :     Herbal immunomodulator for weaned goat-kids
• mRNA Multiplex TaqMan � probe-based RT-PCR:   :     for detection of live MAP bacilli in fecal samples
• Duplex Real-Time PCR:   :     for quick detection of cryptosporidiosis in goats
• Peptide based ETX iELISA (EnteroLISA-ETTM):   :     for assessing protective antibody titre against enterotoxaemia in goats
• Peptide based Indirect ELISA:   :     for early detection of coenurosis in goats

Herbal Products Developed

• Bruclear: It prevents shedding of Brucella melitensis, and treats brucellosis caused by Brucella melitensis in small ruminants.
• Sanjeevani: Herbal immunomodulator for weaned goat-kids .
• Easy Kidder: It ameliorates pregnancy stress in animals .
• Stressol - G: It ameliorates heat & cold stress in animals.

Other Important Technologies Developed

Annual Health Calendar:   :  Preventive health calendar for goats

Faecal Consistency Score Chart   :  for parasitic control in goats

Bruclear:   :  It prevents shedding of Brucella melitensis, and treats brucellosis caused by Brucella melitensis in small ruminants

Easy Kidder (Herbal Drug):  :  It ameliorates pregnancy stress in animals

Stressol�G (Herbal Drug):   : It ameliorates heat & cold stress in animals

BruLISA:   :  IgG and IgM based indirect ELISA for detection of Brucella melitensis

BruCheck:   :  Dot-ELISA for quick and on-spot detection of Brucella melitensis

BruTECT-SRTM:   :  OMP31 TaqMan� probe-based RT-PCR for specific and quick detection of Brucella melitensis

Visual LAMP Assay   :  for quick detection of Brucella melitensis

Latex Milk Agglutination Test:   :  A field test for rapid diagnosis of Johne�s disease (JD)

Indigenous Dot-Elisa:   : A sensitive field-based herd screening test for JD

Nano-Immuno Test   :  for early and rapid detection of live Mycobacterium avium subspecies paratuberculosis (MAP) bacilli in milk samples

Novel Mce-Truncated Protein based ELISA   :  for diagnosis of JD

Indirect Fluorescent Antibody Test:   :  A milk screening test for JD

Visual LAMP   :  Assay for quick diagnosis of JD

IgG based Indirect ELISA   :  for diagnosis of JD

DIVA ELISA   : for differentiation of JD affected animals from vaccinated ones

ToxiScreen ProTM:   :  Sandwich ELISA that can directly detect presence of epsilon toxin in suspected live and dead animals

bfpA gene-based RT-PCR   :  for detection of enteropathogenic E. coli

TaqMan� probe-based RT-PCR   :  for simultaneous detection of Group-A Rota and Bovine Corona viruses

Conventional PCR   :  for quick detection of contagious ecthyma

mRNA based TaqMan� PCR   : for detection and differentiation of live thin walled and thick-walled oocysts of Cryptosporidium species

Plaque Assay   : for titration of PPR virus

Process to purify PPR and FMD viruses   :  from the mixture of different viruses

Primary Goat Testes Cell Culture for quick isolation of Orf virus   : 

DNA Extraction Protocol   :  for Cryptosporidium spp. oocysts present in fecal samples of goats

Nucleotide Databases   :  for ET caused by Clostridium perfringens Type D

Vaccines Developed

• Indigenous JD Vaccine (BioJD Gel ® and BioJD Oil®): It is killed vaccine, and was developed using " Indian Bison Type" genotype of Mycobacterium avium subspecies paratuberculosis strain " S5" of goat origin.
• Killed Vaccine against colibacillosis that provides passive lacteal protection in neonatal goat-kids.
• Currently, the work is going on the novel recombinant chimeric multi-epitope vaccine against Clostridium perfringens Type D causing enterotoxaemia in domestic ruminants.

Product/Method/Process/Procedure/Database Developed

• Plaque Assay for titration of PPR virus.
• Process to purify PPR and FMD viruses from the mixture of different viruses.
• Primary Goat Testes Cell Culture for quick isolation of Orf virus.
• DNA Extraction Protocol: for Cryptosporidium spp. oocysts present in fecal samples of goats .
• E. coli Phage-Cocktail for treatment of colibacillosis in goat-kids.
• Standard Operating Procedure for management of newborn goat-kids.
• Annual Health Calendar: Preventive Health Calendar for Goats.
• Para-Check Card: An eye mucosa color based targeted selective treatment chart against haemonchosis in goats .
• Faecal Consistency Score Chart for parasitic control in goats.
• Nucleotide Databases for ET caused by Clostridium perfringens Type D.

Recognitions/Awards

• DBT Fellowship for Research at Wisconsin University, USA.
• Japan International Cooperation Agency Fellowship.
• Visiting Scientist to USDA, USA.
• Post-Doctoral Research in Grenada, West Indies.
• National Research Development Corporation Award-2014 .
• Expert Member of Empowered Committee on Animal Health, GoI.
• Expert Member of Central Drugs Standard Control Organization, GoI .
• Board Member of International Goat Association representing Asia.
• Board Member of International Association of Para-TB.
• Fellowships (n=2) received from National Academy of Veterinary Sciences.
• Fellowships (n=5) received from Professional Societies ( ISSPGU, ISVM, ISVP & VIPM ).



Name of the Scientist	Designation	Discipline	E-mail address	EPBAX No.	View

1	Dr. K. Gururaj	Veterinary Microbiology	Principal Scientist (Head)	k.gururaj@icar.org.in	132	View
2	Dr.Nitika Sharma	Veterinary Medicine	Senior Scientist	nitika.sharma@icar.gov.in	130	View
2	Dr. A. K. Mishra	Veterinary Microbiology	Senior.Scientist	anil.mishra79@icar.org.in	--	View
4	Dr. Nisha Bisht	Veterinary Parasitology	Scientist	nisha.bisht27@icar.org.in	128	View